Cyclooxygenase-2 expression in uveal melanoma: novel classification of mixed-cell-type tumours

Can J Ophthalmol. 2003 Aug;38(5):352-6. doi: 10.1016/s0008-4182(03)80045-5.

Abstract

Background: The expression of cyclooxygenase-2 (COX-2), an inducible prostaglandin (PG) synthase, has been investigated in various human malignant diseases, such as cutaneous melanoma. We investigated the expression of COX-2 in uveal melanoma and related the findings to prognostic factors.

Methods: In 40 cases of uveal melanoma, immunostaining for COX-2 was done. COX-2 expression was related to histopathological prognostic markers, such as cell type, the presence of lymphocytic infiltration and vascular closed loops in the tumour, and cytomorphometry results.

Results: COX-2 expression was found in 58% of the cases, and it correlated with markers of poor prognosis, such as epithelioid cell type and the presence of lymphocytic infiltration and vascular closed loops. The uveal melanomas expressing COX-2 had larger nuclei, as determined by cytomorphometry.

Interpretation: Whereas epithelioid tumours carry a worse prognosis than spindle cell tumours, until now it has not been possible to give a strong indication of prognosis in mixed-cell tumours. This study showed that mixed-cell tumours, representing the majority of uveal melanomas, may be further subclassified according to COX-2 expression, which serves as a marker of poor prognosis. The role of COX-2 in uveal melanoma should be further elucidated, and the use of COX-2 inhibitors warrants investigation as adjuvant treatment for this life-threatening malignant disease.

MeSH terms

  • Biomarkers, Tumor / metabolism
  • Cell Nucleus / ultrastructure
  • Cyclooxygenase 2
  • Humans
  • Immunologic Techniques
  • Isoenzymes / metabolism*
  • Lymphocytes / pathology
  • Melanoma / enzymology*
  • Melanoma / pathology
  • Membrane Proteins
  • Microscopy, Electron
  • Prognosis
  • Prostaglandin-Endoperoxide Synthases / metabolism*
  • Staining and Labeling
  • Uveal Neoplasms / enzymology*
  • Uveal Neoplasms / pathology

Substances

  • Biomarkers, Tumor
  • Isoenzymes
  • Membrane Proteins
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • Prostaglandin-Endoperoxide Synthases