Abstract
To characterize the features of JAK/STAT signaling in Drosophila immune response, we have identified totA as a gene that is regulated by the JAK/STAT pathway in response to septic injury. We show that septic injury triggers the hemocyte-specific expression of upd3, a gene encoding a novel Upd-like cytokine that is necessary for the JAK/STAT-dependent activation of totA in the Drosophila counterpart of the mammalian liver, the fat body. In addition, we demonstrate that totA activation also requires the NF-KB-like Relish pathway, indicating that fat body cells integrate the activity of NF-KB and JAK/STAT signaling pathways upon immune response. This study reveals that, in addition to the pattern recognition receptor-mediated NF-KB-dependent immune response, Drosophila undergoes a complex systemic response that is mediated by the production of cytokines in blood cells, a process that is similar to the acute phase response in mammals.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adipocytes / cytology
-
Adipocytes / metabolism
-
Animals
-
Blotting, Northern
-
Bridged-Ring Compounds / metabolism
-
Cloning, Molecular
-
DNA-Binding Proteins / metabolism
-
DNA-Binding Proteins / physiology*
-
Drosophila
-
Drosophila Proteins / metabolism
-
Fat Body / metabolism
-
Female
-
Gene Expression Regulation, Developmental
-
Hemocytes / metabolism
-
Hemocytes / physiology*
-
Immunohistochemistry
-
In Situ Hybridization
-
Infections
-
Insect Proteins / metabolism
-
Male
-
Membrane Proteins / metabolism
-
Protein-Tyrosine Kinases / physiology*
-
RNA, Messenger / biosynthesis
-
Receptors, Interleukin / metabolism
-
Reverse Transcriptase Polymerase Chain Reaction
-
Sequence Analysis, Protein
-
Signal Transduction / physiology*
-
Time Factors
-
Trans-Activators / physiology*
-
Transcription Factors / metabolism
-
Transgenes
Substances
-
Bridged-Ring Compounds
-
DNA-Binding Proteins
-
Drosophila Proteins
-
Insect Proteins
-
Membrane Proteins
-
RNA, Messenger
-
Receptors, Interleukin
-
Rel protein, Drosophila
-
Trans-Activators
-
Transcription Factors
-
dome protein, Drosophila
-
Protein-Tyrosine Kinases
-
tetramethylenedisulfotetramine