Characterization of a cDNA clone encoding molluscan insulin-related peptide V of Lymnaea stagnalis

Brain Res Mol Brain Res. 1992 Jun;14(1-2):7-12. doi: 10.1016/0169-328x(92)90003-t.

Abstract

A cDNA clone encoding molluscan insulin-related peptide V (MIP V) was isolated from a cDNA library of the central nervous system (CNS) of the freshwater snail, Lymnaea stagnalis, using a heterologous screening with a previously identified MIP II cDNA. The MIP V cDNA encodes a preprohormone resembling the organization of preproinsulin, with a putative signal sequence, and an A and B chain, however, in this case connected by two distinct C peptide, C alpha and C beta, instead of one single C peptide. This phenomenon, which is shared by the MIP II precursor, represents a new development in the prohormone organization of peptides belonging to the insulin superfamily. The A and B chains of MIPs V, I and II, differ remarkably in primary structure; in contrast, the C alpha peptide domains are almost identical. MIP V has only limited sequence similarity with insulins and related peptides. Both MIP V and I exhibit structural features, which make them a unique class of the insulin superfamily. The MIP I, II and V genes are expressed in a single type of neuron: the growth controlling neuroendocrine light green cells of the Lymnaea CNS.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA / genetics*
  • Genes
  • Invertebrates / genetics
  • Lymnaea / genetics*
  • Molecular Sequence Data
  • Multigene Family
  • Neuropeptides / genetics*
  • Protein Precursors / genetics
  • Sequence Homology, Nucleic Acid
  • Vertebrates / genetics

Substances

  • Neuropeptides
  • Protein Precursors
  • insulin-related neuropeptide
  • DNA

Associated data

  • GENBANK/L01279
  • GENBANK/S42167
  • GENBANK/S42168
  • GENBANK/S72766
  • GENBANK/S72767
  • GENBANK/S72768
  • GENBANK/S72769
  • GENBANK/S72771
  • GENBANK/X59302
  • GENBANK/X63098