The maintenance of hippocampal long-term potentiation is paralleled by a dopamine-dependent increase in glycoprotein fucosylation

Neurochem Int. 1992 Oct;21(3):403-8. doi: 10.1016/0197-0186(92)90191-s.

Abstract

Induction of long-term potentiation (LTP) in hippocampal slices of rats caused an increase in both protein synthesis and glycoprotein fucosylation by 38 and 34%, respectively. The enhanced incorporation of [3H]fucose into glycoproteins observed 1 h after tetanization was abolished in the presence of the dopamine D1 receptor antagonist SCH23390 during stimulation whereas the LTP-induced increase of protein synthesis was not influenced by this drug. The enhanced insertion of [3H]fucose into hippocampal glycoproteins 1 h after tetanization was paralleled by an increase in the activity of the fucose metabolizing enzyme, fucokinase. In contrast no changes in protein and glycoprotein synthesis were detectable 5 h after tetanization of the slices. The results provide evidence that in addition to an enhanced protein synthesis a dopamine (D1) mediated increase in glycoprotein fucosylation is necessary for the maintenance of the late stage of LTP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzazepines / pharmacology*
  • Carbon Radioisotopes
  • Dopamine / physiology*
  • Electric Stimulation
  • Fucose / metabolism*
  • Glycoproteins / biosynthesis*
  • Hippocampus / drug effects
  • Hippocampus / metabolism
  • Hippocampus / physiology*
  • In Vitro Techniques
  • Kinetics
  • Leucine / metabolism
  • Male
  • Membrane Potentials / drug effects
  • Nerve Tissue Proteins / biosynthesis*
  • Phosphotransferases (Alcohol Group Acceptor)*
  • Phosphotransferases / metabolism
  • Pyramidal Tracts / drug effects
  • Pyramidal Tracts / metabolism
  • Pyramidal Tracts / physiology
  • Rats
  • Rats, Wistar
  • Receptors, Dopamine D1 / antagonists & inhibitors*
  • Time Factors
  • Tritium

Substances

  • Benzazepines
  • Carbon Radioisotopes
  • Glycoproteins
  • Nerve Tissue Proteins
  • Receptors, Dopamine D1
  • Tritium
  • Fucose
  • Phosphotransferases
  • Phosphotransferases (Alcohol Group Acceptor)
  • fucokinase
  • Leucine
  • Dopamine