Analysis of the 5' flanking sequence of the G gamma globin gene by denaturing gradient gel electrophoresis confirms the heterogeneity of the Bantu beta S haplotype

Hum Genet. 1992 Sep-Oct;90(1-2):23-6. doi: 10.1007/BF00210740.

Abstract

The GC-->TT polymorphism recently described at positions -1106 and -1105 in the 5' flanking region of the G gamma globin gene for the Bantu beta S haplotype was analysed by denaturing gradient gel electrophoresis. We studied 108 beta S chromosome and 122 beta A chromosomes. The TT sequence was found as follows: in all of 80 chromosomes bearing the Bantu beta S haplotype with the 6-bp deletion -400 nt from the G gamma gene in 3 out of 5 Bantu beta S chromosomes without the deletion, in 1 out of 122 beta A chromosomes from different ethnic origins but in none of 23 beta S chromosomes bearing the Senegal, Benin or Cameroon haplotypes. These results confirm the heterogeneity of the Bantu beta S haplotype and allow a tentative evolutionary sequence for the different alleles at this locus to be presented.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anemia, Sickle Cell / genetics*
  • Base Sequence
  • Electrophoresis
  • Globins / genetics*
  • Haplotypes
  • Humans
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / genetics
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length*

Substances

  • Oligodeoxyribonucleotides
  • Globins