Abstract
A DNA construct containing the human alpha 1-antitrypsin gene including 1.5 and 4 kb of 5' and 3' flanking sequences, was microinjected into the pronucleus of rabbit embryos. The recombinant human protein was (a) expressed in the blood circulation of F0 and F1 transgenic rabbits at an average concentration of 1 mg ml-1, (b) shown to be fully active and (c) shown to be separable from its rabbit counterpart. Transgenic rabbits might represent a novel source of human proteins of therapeutic interest.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified
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Blotting, Northern
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DNA / blood
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Embryo Transfer
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Female
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Humans
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Microinjections
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Pancreatic Elastase / antagonists & inhibitors
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Rabbits
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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alpha 1-Antitrypsin / biosynthesis*
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alpha 1-Antitrypsin / genetics
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alpha 1-Antitrypsin / isolation & purification
Substances
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Recombinant Proteins
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alpha 1-Antitrypsin
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DNA
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Pancreatic Elastase