The effect of Pluronic F-68 on hybridoma cells in continuous culture

Appl Microbiol Biotechnol. 1992 Apr;37(1):44-5. doi: 10.1007/BF00174201.

Abstract

The comparative effects of Pluronic on cell growth of hybridomas were studied. The addition of 0.05% Pluronic decreased the steady-state cell number in continuous culture by about 12% compared to a non-supplemented culture. In short-term experiments, results demonstrated a gradual decrease in cell number with increasing concentration of Pluronic. Such growth inhibition was found to be a result of lowering the rate of DNA synthesis.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Hybridomas / drug effects*
  • Mice
  • Poloxalene / pharmacology*

Substances

  • Poloxalene