UV absorption complicates PCR decontamination

Biotechniques. 1992 Aug;13(2):208-10.

Abstract

UV irradiation is widely used to inactivate contaminating DNA in PCR. Highly UV-absorbent deoxyribonucleoside triphosphates in PCR mixtures reduce the efficiency of UV decontamination. Optimal decontamination may be achieved by irradiating the PCR mixture without the deoxyribonucleoside triphosphates.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Artifacts*
  • Base Sequence
  • DNA / radiation effects*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Ultraviolet Rays*

Substances

  • DNA