Characterization of a novel T lymphocyte protein which binds to a site related to steroid/thyroid hormone receptor response elements in the negative regulatory sequence of the human immunodeficiency virus long terminal repeat

Nucleic Acids Res. 1992 Oct 25;20(20):5429-34. doi: 10.1093/nar/20.20.5429.

Abstract

We have previously identified a T lymphocyte protein which binds to a site within the LTR of the human immunodeficiency virus type 1 (HIV-1) and exerts an inhibitory effect on virus gene expression. The palindromic site (site B) recognized by this protein is related to the palindromic binding sites of members of the steroid/thyroid hormone receptor family. Here we characterize the T cell protein binding to this site as a 100 kD protein which is most abundant in T cells and which binds to site B as a 200 kD complex. This protein is distinct from other members of the steroid/thyroid hormone receptor family including the COUP protein which has a closely related DNA binding specificity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites / genetics
  • Blotting, Northern
  • COUP Transcription Factor I
  • DNA-Binding Proteins / metabolism
  • HIV Long Terminal Repeat*
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Oligonucleotide Probes / metabolism*
  • Receptors, Thyroid Hormone / metabolism
  • Regulatory Sequences, Nucleic Acid*
  • Repressor Proteins / metabolism*
  • T-Lymphocytes / metabolism*
  • Transcription Factors / metabolism

Substances

  • COUP Transcription Factor I
  • DNA-Binding Proteins
  • NR2F1 protein, human
  • Oligonucleotide Probes
  • Receptors, Thyroid Hormone
  • Repressor Proteins
  • Transcription Factors