Abstract
alpha-Hemoglobin fragments alpha-(133-141), alpha-(134-141), alpha-(135-141), alpha-(137-141), alpha-(134-140), alpha-(133-138), alpha-(134-140) and alpha-(137-138) stimulate L929 tumor cell proliferation, alpha-(134-141) being the most active. alpha-(134-141) stimulates proliferation of M3 melanoma cells, murine embryonic fibroblasts, primary cultures of red bone marrow and spleen cells. In L929 cells the effect of alpha-(134-141) is cell density independent; in M3 cells alpha-(137-141) and alpha-(134-141) are most active at density 10,000 cells/well (96 well plate) independently on FBS content.
MeSH terms
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Animals
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Bone Marrow Cells / drug effects
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Cell Count
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Cell Division / drug effects*
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Cell Survival / drug effects
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Cells, Cultured
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Culture Media, Serum-Free / pharmacology
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Data Interpretation, Statistical
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Dose-Response Relationship, Drug
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Endorphins / pharmacology*
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Globins / chemistry
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Hemoglobins / chemistry*
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Mice
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Oligopeptides / chemical synthesis
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Oligopeptides / pharmacology
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Peptide Fragments / pharmacology*
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Spleen / cytology
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Time Factors
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Tumor Cells, Cultured / drug effects
Substances
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Culture Media, Serum-Free
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Endorphins
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Hemoglobins
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Oligopeptides
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Peptide Fragments
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neo-kyotorphin
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Globins