Fluorescein transport through the blood-aqueous and blood-retinal barriers in diabetic macular edema

Curr Eye Res. 2003 Oct;27(4):247-52. doi: 10.1076/ceyr.27.4.247.16596.

Abstract

Purpose: To investigate the integrity of the blood-aqueous barrier (BAB) and the blood-retinal barrier (BRB) in diabetic patients with clinically significant macular edema (CSME).

Methods: The rate constant (Kd(F)) of the BAB and the permeability of the blood-retinal barrier for non-protein bound fluorescein were determined simultaneously by fluorometry. Results were analysed pairwise in diabetic patients (n = 25) with CSME in one eye and without CSME in the other eye.

Results: Kd(F) for the eyes with CSME was significantly increased compared to eyes without CSME (444. 10(-6) min( -1) and 387. 10(-6) min(-1) respectively, p = 0.01). The passive permeability of the BRB was also significantly increased in CSME (5.7 nm/sec and 3.5 nm/sec respectively, p = 0.009, n = 19).

Conclusions: Both the rate constant of the BAB and the BRB permeability were significantly increased in CSME indicating that eye-specific factors are common for both barriers in diabetic patients with CSME.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Blood-Aqueous Barrier / metabolism*
  • Blood-Retinal Barrier / metabolism*
  • Capillary Permeability
  • Contrast Media / pharmacokinetics*
  • Diabetic Retinopathy / metabolism*
  • Diabetic Retinopathy / physiopathology
  • Edema / metabolism*
  • Edema / physiopathology
  • Fluorescein / pharmacokinetics*
  • Humans
  • Macula Lutea*
  • Middle Aged
  • Visual Acuity

Substances

  • Contrast Media
  • Fluorescein