NF-kappa B-binding activity in an animal diet-induced overweightness model and the impact of subsequent energy restriction

Biochem Biophys Res Commun. 2003 Nov 14;311(2):533-9. doi: 10.1016/j.bbrc.2003.10.028.

Abstract

An impaired immune function linked to obesity has been shown in both human and animal studies. The purpose of this work was to analyse the hypothesis that PPAR gamma 1 participates in the inhibition of the immune response by affecting the DNA-binding ability of the NF-kappa B complex and whether the SREBP-1 expression can regulate PPAR gamma 1 expression in spleen. Diet-induced overweight rats showed higher PPAR gamma 1 (p<0.05) and lower SREBP-1 (p<0.01) mRNA expression levels with an inhibition of the DNA-binding ability of NF-kappa B compared to control rats as determined by gel-shift analysis. On the other hand, energy restriction decreased SREBP-1 (p<0.01) mRNA expression with no differences in PPAR gamma 1 mRNA expression compared to non-restricted rats, which was accompanied by a restoration in the DNA-binding ability of NF-kappa B as shown by gel-shift analysis. These results suggest that PPAR gamma 1 may be involved in the altered immune response through changes in the activity of NF-kappa B.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Weight / physiology
  • CCAAT-Enhancer-Binding Proteins / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Diet, Reducing / methods
  • Energy Intake / physiology*
  • Male
  • NF-kappa B / metabolism*
  • Obesity / metabolism
  • Obesity / therapy*
  • Rats
  • Rats, Wistar
  • Receptors, Cytoplasmic and Nuclear / metabolism*
  • Reference Values
  • Spleen
  • Sterol Regulatory Element Binding Protein 1
  • Transcription Factors / metabolism*

Substances

  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • NF-kappa B
  • Receptors, Cytoplasmic and Nuclear
  • Srebf1 protein, rat
  • Sterol Regulatory Element Binding Protein 1
  • Transcription Factors