Abstract
Among Escherichia coli strains isolated from stool specimens from patients with acute diarrhea, 1.4% were found to harbor cdtB by use of enrichment cytolethal distending toxin (CDT) PCR. These isolates were identified as being enteropathogenic E. coli (EPEC). In a retrospective study using a probe hybridization assay, 6 of 138 EPEC strains were found to harbor the cdtB locus. cdtB-positive isolates mostly belong to the O86a and O127a serogroups, with the former being associated with higher expression of CDT. Pulsed-field gel electrophoresis profiles showed that the EPEC strains harboring cdtB strains are genetically diverse.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Acute Disease
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Bacterial Toxins / classification
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Bacterial Toxins / genetics*
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Bacterial Toxins / isolation & purification
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Base Sequence
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Blood / microbiology
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DNA Primers
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Dehydration / microbiology
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Diarrhea / microbiology*
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Electrophoresis, Gel, Pulsed-Field
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Escherichia coli / classification
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Escherichia coli / genetics
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Escherichia coli / isolation & purification
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Escherichia coli / pathogenicity*
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Escherichia coli Infections / blood
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Escherichia coli Infections / microbiology*
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Escherichia coli Proteins / genetics*
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Escherichia coli Proteins / isolation & purification
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Feces / microbiology*
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Humans
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India
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Molecular Sequence Data
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Occult Blood
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Phylogeny
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Polymerase Chain Reaction
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Serotyping / methods
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Vomiting / microbiology
Substances
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Bacterial Toxins
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DNA Primers
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Escherichia coli Proteins
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cytolethal distending toxin
Associated data
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GENBANK/AY351905
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GENBANK/AY351906
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GENBANK/AY351907