Transductional and transcriptional targeting of cancer cells using genetically engineered viral vectors

Cancer Lett. 2003 Nov 25;201(2):165-73. doi: 10.1016/j.canlet.2003.07.003.

Abstract

Gene delivery vectors, including adenovirus (Ad) and adeno-associated virus (AAV), are inefficient and non-selective for cancer due to low levels of viral receptors with high levels on other tissues, including liver. We tested Ads and AAVs with the SIGYPLP-targeting peptide inserted into virus capsids for transduction in a panel of cancer cells. Six of twelve lines (C8161, PC-3, G-CCM, MKN-45, LnCAP and A549) were transduced, independently of native viral tropism. Furthermore the candidate cancer gene therapy promoter FLT-1 was active in three of these six cell lines. This offers the potential for dual targeting of selected cancer cells.

MeSH terms

  • Adenoviridae / genetics*
  • Dependovirus / genetics*
  • Genetic Engineering
  • Genetic Therapy / methods
  • Genetic Vectors*
  • Humans
  • Neoplasms / metabolism
  • Neoplasms / therapy*
  • Promoter Regions, Genetic
  • Proteinase Inhibitory Proteins, Secretory
  • Proteins / genetics
  • Proteins / metabolism
  • Receptors, Virus / metabolism
  • Transcription, Genetic*
  • Transduction, Genetic*
  • Tumor Cells, Cultured

Substances

  • Proteinase Inhibitory Proteins, Secretory
  • Proteins
  • Receptors, Virus