Methanol production is enhanced by expression of an Aspergillus niger pectin methylesterase in tobacco cells

Tomohisa Hasunuma; Ei-ichiro Fukusaki; Akio Kobayashi

doi:10.1016/j.jbiotec.2003.07.008

Methanol production is enhanced by expression of an Aspergillus niger pectin methylesterase in tobacco cells

J Biotechnol. 2003 Dec 5;106(1):45-52. doi: 10.1016/j.jbiotec.2003.07.008.

Authors

Tomohisa Hasunuma¹, Ei-ichiro Fukusaki, Akio Kobayashi

Affiliation

¹ Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita 565-0871, Japan.

PMID: 14636709
DOI: 10.1016/j.jbiotec.2003.07.008

Abstract

Tobacco suspension culture cell (Nicotiana tabacum, BY2) was transformed with an Aspergillus niger pectin methylesterase (PME; EC 3.1.1.11) cDNA under the control of cauliflower mosaic virus (CaMV) 35S promoter. The transformant indicated a significant rise of PME and the level of methanol in the transformant increased by 28.7% compared to the vector control transformant. This is the first report of methanol overproduction in plant cells by means of genetic engineering.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Aspergillus niger / enzymology*
Aspergillus niger / genetics*
Carboxylic Ester Hydrolases / genetics*
Carboxylic Ester Hydrolases / metabolism*
Cells, Cultured
Gene Expression Regulation, Enzymologic / physiology
Gene Expression Regulation, Plant / physiology
Genetic Enhancement / methods*
Methanol / isolation & purification
Methanol / metabolism*
Nicotiana / genetics*
Nicotiana / metabolism*
Plants, Genetically Modified / metabolism
Protein Engineering / methods
Recombinant Proteins / biosynthesis
Transfection / methods

Substances

Recombinant Proteins
Carboxylic Ester Hydrolases
pectinesterase
Methanol