Highly efficient conversion of lactate to pyruvate using whole cells of Acinetobacter sp

Biotechnol Prog. 2003 Nov-Dec;19(6):1672-6. doi: 10.1021/bp0341242.

Abstract

On an industrial scale, the production of pyruvate at a high concentration from the cheaper lactate substrate is a valuable process. To produce pyruvate from lactate by whole cells, various lactate-utilizing microorganisms were isolated from soil samples. Among them, strain WLIS, identified as Acinetobacter sp., was screened as a pyruvate producer. For the pyruvate preparation from lactate, the preparative conditions were optimized with whole cells of the strain. The cells cultivated in the medium containing 100 mM of l-lactate showed the highest biotransformation efficiency from lactate to pyruvate. The optimized dry-cell concentration, pH, and temperature of reaction were 6 g/L, pH 7.0-7.5, and 30 degrees C, respectively. The influences of ethylenediaminetetraacetic acid (EDTA) and aeration on a biotransformation reaction were carried out under the test conditions. Under the optimized reaction conditions, l-lactate at concentrations of 200 and 500 mM were almost totally stoichiometrically converted into pyruvate in 8 and 12 h, respectively. About 60% of 800 mM of l-lactate was transformed into pyruvate in 24 h. This reduced conversion rate is probably due to the high substrate inhibition in biotransformation.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter / cytology
  • Acinetobacter / growth & development*
  • Acinetobacter / isolation & purification
  • Acinetobacter / metabolism*
  • Bioreactors / microbiology*
  • Biotransformation
  • Cell Culture Techniques / methods*
  • Cell Division / physiology
  • Hydrogen-Ion Concentration
  • Lactic Acid / metabolism*
  • Mixed Function Oxygenases / metabolism*
  • Pilot Projects
  • Pyruvic Acid / isolation & purification
  • Pyruvic Acid / metabolism*
  • Temperature

Substances

  • Lactic Acid
  • Pyruvic Acid
  • Mixed Function Oxygenases
  • lactate 2-monooxygenase