[Construction of N-LMP1 transgenic mice with the specific regulation region in nasopharynx]

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2003 Dec;35(12):1072-6.
[Article in Chinese]

Abstract

In order to elucidate the role of EBV-LMP1 in the nasopharyngeal carcinogenesis, the expression vector was constructed with subjecting the N-LMP1 gene to double regulation of two specific regulators: EDL-2 and PLUNC-p. The N-LMP1 related transgenic mice model has been constructed successfully by pronucleus microinjection. 58 founder mice were born, 4 of which were founded to be positive by PCR and Southern blot. Immunohistochemistry assay showed that N-LMP1 protein was expressed in the nasopharynx, tongue and forestomach of transgenic mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Female
  • Gastric Mucosa / metabolism
  • Gene Expression Regulation, Viral
  • Humans
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Transgenic
  • Nasopharynx / chemistry
  • Nasopharynx / metabolism*
  • Regulatory Sequences, Nucleic Acid / genetics*
  • Stomach / chemistry
  • Tongue / chemistry
  • Tongue / metabolism
  • Viral Matrix Proteins / analysis
  • Viral Matrix Proteins / genetics*

Substances

  • EBV-associated membrane antigen, Epstein-Barr virus
  • Viral Matrix Proteins