Abstract
Recent studies have implicated a possible role for NK cells in regulating dendritic cells (DC) in vitro. In the present study, we demonstrate that immature DC are rapidly eliminated by NK cells in vivo via a pathway dependent on the TNF-related apoptosis-inducing ligand (TRAIL). Elimination of NK cells and/or neutralization of TRAIL function during immunization with immature DC loaded with nonself or tumor Ags significantly enhanced T cell responses to these Ags and Ag-specific tumor immunity. These data suggested that NK cell TRAIL might regulate responses to vaccination by controlling the survival of Ag-loaded DC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / immunology*
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Apoptosis Regulatory Proteins
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Bone Marrow Cells / cytology
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Bone Marrow Cells / immunology
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Cancer Vaccines / administration & dosage*
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Cancer Vaccines / immunology*
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Cell Differentiation / immunology
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Cell Line, Tumor
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Cell Survival / immunology
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Cells, Cultured
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Cytotoxicity Tests, Immunologic
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Cytotoxicity, Immunologic* / genetics
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Dendritic Cells / cytology
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Dendritic Cells / immunology
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Dendritic Cells / transplantation*
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Graft Rejection / immunology
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Growth Inhibitors / deficiency
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Growth Inhibitors / genetics
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Growth Inhibitors / physiology*
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Killer Cells, Natural / cytology
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Killer Cells, Natural / immunology*
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Killer Cells, Natural / metabolism
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Ligands
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Lymphocyte Depletion
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Membrane Glycoproteins / deficiency
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / physiology*
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Neoplasm Transplantation
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TNF-Related Apoptosis-Inducing Ligand
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Tumor Necrosis Factor-alpha / deficiency
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / physiology*
Substances
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Apoptosis Regulatory Proteins
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Cancer Vaccines
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Growth Inhibitors
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Ligands
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Membrane Glycoproteins
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TNF-Related Apoptosis-Inducing Ligand
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Tnfsf10 protein, mouse
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Tumor Necrosis Factor-alpha