Abstract
Ubiquitin-dependent proteolysis of cellular proteins is one of the major pathways to regulate protein function posttranslationally. Here we demonstrate a potentially general method of degrading any targeted proteins by the ubiquitin-dependent proteolysis in living cells, using small-molecule proteolysis inducer (SMPI).
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit
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Multienzyme Complexes / chemistry
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Multienzyme Complexes / metabolism*
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Peptide Fragments / chemistry
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Peptide Fragments / metabolism*
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Peptide Hydrolases / chemistry
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Peptide Hydrolases / metabolism*
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Proteins / chemistry
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Proteins / metabolism*
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Transcription Factors / metabolism
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Tumor Cells, Cultured
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Ubiquitin / chemistry
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Ubiquitin / metabolism*
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Ubiquitin-Protein Ligases / metabolism
Substances
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HIF1A protein, human
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Hypoxia-Inducible Factor 1, alpha Subunit
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Multienzyme Complexes
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Peptide Fragments
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Proteins
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Transcription Factors
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Ubiquitin
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Ubiquitin-Protein Ligases
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Peptide Hydrolases