23Na NMR evaluation of human erythrocytes Na+/K+/CL-cotransport. A study in elderly hypertensives

Cell Mol Biol (Noisy-le-grand). 1992 Dec;38(8):877-84.

Abstract

The behaviour of sodium transport systems across the cell membrane has been poorly investigated in elderly hypertension. Sodium efflux driven by Na+/K+/Cl-cotransport activity was therefore investigated (using a novel NMR-spectroscopy method) in 5 elderly hypertensive males (mean age 78 +/- 5 years) and 5 normotensive controls (mean age 79 +/- 3 years). In order to exclude any change in cotransport activity secondary to metabolic abnormalities, both patients and controls were non-obese and had normal glucose and lipid metabolisms. The Na+/K+/Cl-cotransport evaluation was performed after three months of pharmacological wash-out, under a diet containing 120 mEq of Na+/day. The resulting data showed that Na+ efflux due to outward Na+/K+/Cl-cotransport was higher in hypertensive group than in the normotensive one (0.50 +/- 0.10 mmol Na+/l cells/hr. vs 0.33 +/- 0.03 mmol Na+/l cells/hr., respectively, p < 0.05). Intracellular Na+ content was similar in both groups. At variance with previous data from the literature, our findings indicate that the Na+/K+/Cl-cotransport activity is elevated in elderly hypertensives.

Publication types

  • Comparative Study

MeSH terms

  • Aged
  • Aldosterone / blood
  • Blood Pressure
  • Carrier Proteins / blood*
  • Cholesterol / blood
  • Creatinine / blood
  • Erythrocytes / metabolism*
  • Humans
  • Hypertension / blood*
  • Insulin / blood
  • Kinetics
  • Magnetic Resonance Spectroscopy / methods
  • Male
  • Reference Values
  • Renin / blood
  • Sodium / blood*
  • Sodium-Potassium-Chloride Symporters
  • Triglycerides / blood

Substances

  • Carrier Proteins
  • Insulin
  • Sodium-Potassium-Chloride Symporters
  • Triglycerides
  • Aldosterone
  • Cholesterol
  • Sodium
  • Creatinine
  • Renin