Expression of human CD4 in transgenic mice does not confer sensitivity to human immunodeficiency virus infection

AIDS Res Hum Retroviruses. 1992 Dec;8(12):2063-71. doi: 10.1089/aid.1992.8.2063.

Abstract

Transfection of the human CD4 molecule into mouse cells does not confer susceptibility to human immunodeficiency virus type 1 (HIV-1) infection. Expression of the human CD4 molecule in transgenic mice was seen to offer some new possibilities. However, transgenic mouse T cells expressing either the human CD4 receptor, or a hybrid human/mouse CD4 receptor alone or in conjunction with human major histocompatibility complex class I molecules, were refractory to in vitro HIV-1 infection. In addition, no infection was observed after in vivo HIV inoculation to mice of these various transgenic lines. Injection of recombinant gp160 viral protein to the transgenic mice did not alter their T and B cell populations. The existence of a dominant block in mouse cells that prevents HIV entry is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • CD4 Antigens / genetics*
  • DNA, Viral / genetics
  • Disease Models, Animal
  • Gene Expression
  • Gene Products, env / immunology
  • HIV Envelope Protein gp160
  • HIV Infections / etiology*
  • HIV Infections / genetics
  • HIV Infections / immunology
  • HIV-1* / genetics
  • HIV-1* / immunology
  • Humans
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Protein Precursors / immunology

Substances

  • CD4 Antigens
  • DNA, Viral
  • Gene Products, env
  • HIV Envelope Protein gp160
  • Protein Precursors