Human monoclonal antibody fragment specific for glycoprotein G in herpes simplex virus type 2 with applications for serotype-specific diagnosis

J Clin Microbiol. 2004 Mar;42(3):1250-3. doi: 10.1128/JCM.42.3.1250-1253.2004.

Abstract

A combinatorial library was used to select a human monoclonal antibody fragment (Fab) with high affinity for G glycoprotein in herpes simplex virus type 2 (HSV-2). Tests with 112 clinical specimens demonstrated successful discrimination between HSV-2 and HSV-1, showing the potential of Fab as a low-cost tool for HSV subtyping in clinical diagnosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Binding Sites
  • Chlorocebus aethiops
  • Herpes Simplex / diagnosis*
  • Herpesvirus 2, Human / immunology*
  • Herpesvirus 2, Human / isolation & purification
  • Humans
  • Immunoglobulin Fab Fragments / immunology
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Peptide Fragments / immunology
  • Vero Cells
  • Viral Envelope Proteins / immunology*

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin Fab Fragments
  • Peptide Fragments
  • Viral Envelope Proteins
  • glycoprotein B, herpes simplex virus type 2