Abstract
We describe the use of an immunofluorescence assay and coculture to confirm human T-cell leukemia-lymphoma virus (HTLV) infection. Peripheral blood mononuclear cells from 32 of 32 seropositive donors were positive in the immunofluorescence assay, and 63% of their cocultures produced p24 antigen. Specific antibodies distinguished HTLV type I (HTLV-I) from HTLV-II. HTLV-I or HTLV-II was isolated from donors with indeterminate serologic test results.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal
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Blood Donors*
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Enzyme-Linked Immunosorbent Assay
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Evaluation Studies as Topic
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Fluorescent Antibody Technique / statistics & numerical data
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HTLV-I Infections / diagnosis
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HTLV-II Infections / diagnosis
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Human T-lymphotropic virus 1 / immunology
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Human T-lymphotropic virus 1 / isolation & purification*
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Human T-lymphotropic virus 2 / immunology
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Human T-lymphotropic virus 2 / isolation & purification*
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Humans
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Sensitivity and Specificity
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Virology / methods