Phosphorylcholine-containing polymers for use in cell encapsulation

Artif Cells Blood Substit Immobil Biotechnol. 2004 Feb;32(1):91-104. doi: 10.1081/bio-120028671.

Abstract

A model system for encapsulation of pancreatic islets which has potential properties for improving biocompatibility and immunosuppression was investigated. In vitro and in vivo studies have shown that phosphorylcholine-containing polymers have high biocompatibility due to low adsorption of proteins and reduced thrombus formation. Encapsulation of islets isolated from rats with a compound membrane composed of phosphorylcholine-containing polymers and cellulose acetate led to rapid insulin production and diffusion across the membrane in response to glucose challenge. The phosphorylcholine-containing polymer had a molecular weight of about 1.3 x 10(4) Da. The polymer-coated membrane excluded larger molecules such as IgG (molecular weight 150 kDa), thereby acting as a physical immuno-barrier, but allowed smaller molecules such as glucose and insulin to pass through.

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry*
  • Biocompatible Materials / therapeutic use
  • Cell Transplantation
  • Cellulose / analogs & derivatives*
  • Glucose / metabolism
  • Immunoglobulin G / metabolism
  • Immunosuppression Therapy
  • Insulin / metabolism
  • Islets of Langerhans Transplantation / methods*
  • Male
  • Permeability
  • Phosphorylcholine
  • Polymers / therapeutic use*
  • Rats
  • Rats, Wistar

Substances

  • Biocompatible Materials
  • Immunoglobulin G
  • Insulin
  • Polymers
  • Phosphorylcholine
  • acetylcellulose
  • Cellulose
  • Glucose