Abstract
The SKN7 two-component response regulator gene of Candida albicans was deleted, and the phenotype of the mutant was established. This mutant exhibited impaired growth on Spider agar and 10% serum agar compared to wild-type and gene-reconstituted strains. The skn7 mutant was sensitive to H(2)O(2) in vitro, but its virulence was only mildly attenuated. A comparison of the Skn7p and Ssk1p response regulators of C. albicans is discussed.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Candida albicans / genetics
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Candida albicans / growth & development
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Candida albicans / pathogenicity*
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Candida albicans / physiology*
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Candidiasis / microbiology
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Candidiasis / physiopathology*
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Culture Media
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Disease Models, Animal
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Gene Deletion*
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Gene Expression Regulation, Fungal
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Humans
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Hydrogen Peroxide / pharmacology
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Mice
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Oxidative Stress
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Phenotype
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Saccharomyces cerevisiae Proteins*
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Signal Transduction
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Virulence
Substances
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Culture Media
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DNA-Binding Proteins
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Fungal Proteins
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SKN7 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Hydrogen Peroxide