Evaluation of the BDProbeTec strand displacement amplification assay in comparison with the AMTD II direct test for rapid diagnosis of tuberculosis

Clin Microbiol Infect. 2004 Apr;10(4):332-4. doi: 10.1111/j.1198-743X.2004.00818.x.

Abstract

The BDProbeTec MTB assay for direct detection of Mycobacterium tuberculosis was evaluated in comparison with the AMTD-II assay on 94 samples from different patients with clinical suspicion of tuberculosis. Using a combination of culture on Lowenstein-Jensen medium (with or without preculture in BACTEC 9000) and clinical diagnosis as the standard, BDProbeTec MTB showed high sensitivity and specificity (96.1% and 100%, respectively), similar to AMTD-II (96.1% and 97.1%, respectively), with significantly higher sensitivity than the Ziehl-Neelsen stain for acid-fast bacilli (73%, p < 0.05).

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Culture Media
  • DNA Transposable Elements / genetics
  • DNA, Ribosomal / genetics
  • Humans
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / growth & development
  • Mycobacterium tuberculosis / isolation & purification*
  • Nucleic Acid Amplification Techniques / methods*
  • RNA, Ribosomal, 16S / genetics
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Tuberculosis / diagnosis*
  • Tuberculosis / microbiology
  • Tuberculosis, Pulmonary / diagnosis*
  • Tuberculosis, Pulmonary / microbiology

Substances

  • Culture Media
  • DNA Transposable Elements
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S
  • Reagent Kits, Diagnostic