Abstract
A recombinant adenoviral tetracycline-regulated system with neuron-specific enolase (NSE) promoter was injected stereotaxically into the striatum of rat brains. The efficiency of in vivo transfection was quantified by counting the number of green fluorescent protein (GFP)-positive cells at 3 days, 1 week, and 4 weeks after injection. NeuN immunohistochemistry demonstrated that expression of gammaPKC-GFP was dominant (20-99%) in neuron and expression of gammaPKC-GFP in neuron was significantly higher in pups than adult rats. These results indicate that tetracycline-inhibitable transcription factor (tTA) can drive tetracycline-responsive promoter (TetOp) under the control of NSE promoter, thereby efficiently and selectively expressing gammaPKC-GFP in neurons in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / genetics*
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Animals
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Animals, Newborn
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Brain / anatomy & histology
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Brain / cytology
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Brain / metabolism
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Gene Expression / drug effects
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Gene Targeting / methods*
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Genetic Vectors / genetics
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Green Fluorescent Proteins
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Injections
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Luminescent Proteins / genetics
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Male
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Microscopy, Fluorescence
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Neurons / metabolism
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Neurons / physiology*
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Phosphopyruvate Hydratase / genetics*
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Promoter Regions, Genetic / physiology
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Protein Kinase C / biosynthesis
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Protein Kinase C / genetics
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Rats
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Rats, Sprague-Dawley
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Stereotaxic Techniques
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Tetracycline / pharmacology*
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Transgenes / drug effects
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Transgenes / genetics*
Substances
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Luminescent Proteins
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Green Fluorescent Proteins
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protein kinase C gamma
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Protein Kinase C
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Phosphopyruvate Hydratase
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Tetracycline