In vivo transgene expression using an adenoviral tetracycline-regulated system with neuron-specific enolase promoter

Biochem Biophys Res Commun. 2004 May 14;317(4):1144-8. doi: 10.1016/j.bbrc.2004.03.166.

Abstract

A recombinant adenoviral tetracycline-regulated system with neuron-specific enolase (NSE) promoter was injected stereotaxically into the striatum of rat brains. The efficiency of in vivo transfection was quantified by counting the number of green fluorescent protein (GFP)-positive cells at 3 days, 1 week, and 4 weeks after injection. NeuN immunohistochemistry demonstrated that expression of gammaPKC-GFP was dominant (20-99%) in neuron and expression of gammaPKC-GFP in neuron was significantly higher in pups than adult rats. These results indicate that tetracycline-inhibitable transcription factor (tTA) can drive tetracycline-responsive promoter (TetOp) under the control of NSE promoter, thereby efficiently and selectively expressing gammaPKC-GFP in neurons in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Animals, Newborn
  • Brain / anatomy & histology
  • Brain / cytology
  • Brain / metabolism
  • Gene Expression / drug effects
  • Gene Targeting / methods*
  • Genetic Vectors / genetics
  • Green Fluorescent Proteins
  • Injections
  • Luminescent Proteins / genetics
  • Male
  • Microscopy, Fluorescence
  • Neurons / metabolism
  • Neurons / physiology*
  • Phosphopyruvate Hydratase / genetics*
  • Promoter Regions, Genetic / physiology
  • Protein Kinase C / biosynthesis
  • Protein Kinase C / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Stereotaxic Techniques
  • Tetracycline / pharmacology*
  • Transgenes / drug effects
  • Transgenes / genetics*

Substances

  • Luminescent Proteins
  • Green Fluorescent Proteins
  • protein kinase C gamma
  • Protein Kinase C
  • Phosphopyruvate Hydratase
  • Tetracycline