Cloning and sequence of a functionally active cDNA encoding the mouse ubiquitin-activating enzyme E1

Gene. 1992 Sep 10;118(2):279-82. doi: 10.1016/0378-1119(92)90200-9.

Abstract

A cDNA encoding the ubiquitin-activating enzyme, E1, was isolated from the mouse mammary carcinoma cell line, FM3A, and shown to complement mutant mouse cells deficient in the enzyme. The 3495-bp cDNA encodes 1058 amino acids (aa), and shares extensive homology with the human E1 enzyme at both the nucleotide and aa sequence levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA / genetics
  • Ligases / chemistry
  • Ligases / genetics*
  • Mice
  • Molecular Sequence Data
  • Mutation / genetics
  • Sequence Homology, Nucleic Acid
  • Ubiquitin-Activating Enzymes
  • Ubiquitin-Protein Ligases

Substances

  • DNA
  • Ubiquitin-Protein Ligases
  • Ligases
  • Ubiquitin-Activating Enzymes

Associated data

  • GENBANK/D10576
  • GENBANK/M74891
  • GENBANK/M74892
  • GENBANK/M79309
  • GENBANK/M79310
  • GENBANK/M90357
  • GENBANK/S43286
  • GENBANK/S43293
  • GENBANK/S43295
  • GENBANK/S43297