Inactivation of the murine Transferrin Receptor 2 gene using the Cre recombinase: loxP system

Genesis. 2004 May;39(1):38-41. doi: 10.1002/gene.20023.

Abstract

Transferrin Receptor 2 (TfR2) is a key molecule involved in the regulation of iron homeostasis. Mutations in TfR2 lead to type 3 hemochromatosis in humans. We have developed mice with a targeted deletion of TfR2. The Cre-recombinase:loxP system used to create the mice allows both full deletion and tissue-specific deletion of TfR2. The development of these mice will provide new models for type 3 hemochromatosis and assist in determining the role of TfR2 in iron metabolism.

MeSH terms

  • Animals
  • Blotting, Western
  • Gene Silencing*
  • Genetic Vectors
  • Hemochromatosis / genetics
  • Hemochromatosis / metabolism
  • Iron / metabolism
  • Liver / metabolism
  • Mice
  • Mice, Knockout
  • Receptors, Transferrin / genetics
  • Receptors, Transferrin / physiology*
  • Recombinases / genetics*

Substances

  • Receptors, Transferrin
  • Recombinases
  • TFR2 protein, mouse
  • Iron