Collaborative study of antibiotic medium 3 and flow cytometry for identification of amphotericin B-resistant Candida isolates

J Clin Microbiol. 2004 May;42(5):2252-4. doi: 10.1128/JCM.42.5.2252-2254.2004.

Abstract

Center 1 used the National Committee for Clinical Laboratory Standards M27-A2 method and antibiotic medium 3 (AM3) test to determine amphotericin B resistance in 5 of 30 Candida isolates. These isolates were tested at center 2 by AM3 test and flow cytometry (FC). The agreements (C1-C2) were 90% for AM3 test and FC and 73% for the AM3 tests.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Amphotericin B / pharmacology*
  • Antifungal Agents / pharmacology*
  • Candida / drug effects*
  • Candida / isolation & purification
  • Culture Media
  • Drug Resistance, Fungal
  • Flow Cytometry
  • Humans
  • Microbial Sensitivity Tests / methods*
  • Microbial Sensitivity Tests / statistics & numerical data

Substances

  • Antifungal Agents
  • Culture Media
  • Amphotericin B