Comparison of efficiency of ex vivo expansion of whole blood, mononuclear cells and purified CD34+ cells from human umbilical cord blood

Haematologica. 2004 May;89(5):606-7.

Abstract

We used a dual-chamber culture system separated by a dialysis membrane to test the efficiency of expansion of whole cord blood and cell fractions. We found that expansion of progenitor cells was more efficient from whole blood than from purified CD34+ or partially purified mononuclear cell fractions.

Publication types

  • Comparative Study
  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD34 / blood*
  • Cell Culture Techniques / methods
  • Cell Proliferation
  • Fetal Blood / cytology*
  • Fetal Blood / immunology
  • Humans
  • Leukocytes, Mononuclear / cytology*
  • Leukocytes, Mononuclear / immunology

Substances

  • Antigens, CD34