Substitution of 3'-phosphate cap with a carbon-based blocker reduces the possibility of fluorescence resonance energy transfer probe failure in real-time PCR assays

Clin Chem. 2004 Jun;50(6):1080-2. doi: 10.1373/clinchem.2004.033183.
No abstract available

MeSH terms

  • Carbon*
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes / chemistry*
  • Oligonucleotide Probes / chemistry*
  • Organophosphates / chemistry*
  • Polymerase Chain Reaction / methods*
  • Reagent Kits, Diagnostic

Substances

  • Fluorescent Dyes
  • Oligonucleotide Probes
  • Organophosphates
  • Reagent Kits, Diagnostic
  • Carbon