Abstract
Doublesex (DSX) is a transcription factor in Drosophila melanogaster that regulates somatic sexual differentiation. Homologues have been found in diverse metazoans, suggesting a conserved role in development. Here, the expression, purification and crystallization of a novel 63-amino-acid dimerization domain and selenomethionine analogue are described. Native crystals belong to space group P2(1)2(1)2(1) and diffract to a resolution of 1.6 A with synchrotron radiation. Selenomethionine-containing crystals provide anomalous dispersion for phasing.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Crystallization
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Crystallography, X-Ray
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DNA-Binding Proteins / chemistry*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / isolation & purification
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Drosophila Proteins / chemistry*
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Drosophila Proteins / genetics
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Drosophila Proteins / isolation & purification
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Drosophila melanogaster / chemistry*
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Drosophila melanogaster / genetics
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Gene Expression
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Transcription Factors / chemistry*
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Transcription Factors / genetics
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Transcription Factors / isolation & purification
Substances
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DNA-Binding Proteins
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DSX protein, Drosophila
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Drosophila Proteins
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Transcription Factors