Expression, crystallization and preliminary X-ray characterization of the Drosophila transcription factor Doublesex

Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1328-30. doi: 10.1107/S0907444904012223. Epub 2004 Jun 22.

Abstract

Doublesex (DSX) is a transcription factor in Drosophila melanogaster that regulates somatic sexual differentiation. Homologues have been found in diverse metazoans, suggesting a conserved role in development. Here, the expression, purification and crystallization of a novel 63-amino-acid dimerization domain and selenomethionine analogue are described. Native crystals belong to space group P2(1)2(1)2(1) and diffract to a resolution of 1.6 A with synchrotron radiation. Selenomethionine-containing crystals provide anomalous dispersion for phasing.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Crystallization
  • Crystallography, X-Ray
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / isolation & purification
  • Drosophila Proteins / chemistry*
  • Drosophila Proteins / genetics
  • Drosophila Proteins / isolation & purification
  • Drosophila melanogaster / chemistry*
  • Drosophila melanogaster / genetics
  • Gene Expression
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics
  • Transcription Factors / isolation & purification

Substances

  • DNA-Binding Proteins
  • DSX protein, Drosophila
  • Drosophila Proteins
  • Transcription Factors