Enhanced post-source decay and cross-ring fragmentation of oligosaccharides facilitated by conversion to amino derivatives

Rapid Commun Mass Spectrom. 2004;18(13):1513-8. doi: 10.1002/rcm.1506.

Abstract

Post-source decay (PSD) fragmentation of chemically or enzymatically produced aminoglycans has been evaluated through matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Conversion of native glycans to their respective aminoglycan derivatives improved detection sensitivity of the usual fragments and promoted cross-ring fragmentation of linear oligosaccharides, facilitating linkage recognition. The cross-ring fragmentations for both dextrin and dextran oligosaccharides were not limited to the reducing-end glucose moiety, as they were extended throughout the entire molecule. When the amino group was generated for N-glycans derived from three different glycoproteins, an enhancement of PSD was observed, without a significant extent of cross-ring fragmentation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Sugars / chemical synthesis*
  • Amino Sugars / chemistry*
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Glycoproteins / chemistry
  • Molecular Sequence Data
  • Molecular Structure
  • Oligosaccharides / chemistry*
  • Reference Standards
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Amino Sugars
  • Glycoproteins
  • Oligosaccharides