Abstract
Analysis of developmental plasticity of bone marrow-derived cells (BMDCs) is complicated by the possibility of cell-cell fusion. Here we demonstrate that epithelial cells can develop from BMDCs without cell-cell fusion. We use the Cre/lox system together with beta-galactosidase and enhanced green fluorescent protein expression in transgenic mice to identify epithelial cells in the lung, liver, and skin that develop from BMDCs without cell fusion.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bone Marrow Cells / physiology*
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Bone Marrow Transplantation*
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Cell Differentiation
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Cell Fusion*
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Cobra Cardiotoxin Proteins / pharmacology
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Elapid Venoms / pharmacology
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Epithelial Cells / cytology*
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Epithelial Cells / metabolism
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Female
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Green Fluorescent Proteins
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Hepatocytes / cytology
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Hepatocytes / metabolism
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Keratinocytes / cytology
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Keratinocytes / metabolism
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Keratins / analysis
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Luminescent Proteins / metabolism
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Male
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Mice
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Mice, Transgenic
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Muscle Cells / cytology
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Radiation, Ionizing
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Recombinases / metabolism
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Recombination, Genetic
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Reverse Transcriptase Polymerase Chain Reaction
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Stem Cells / cytology
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Stem Cells / physiology*
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X Chromosome
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Y Chromosome
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beta-Galactosidase / biosynthesis
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beta-Galactosidase / genetics
Substances
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Cobra Cardiotoxin Proteins
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Elapid Venoms
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Luminescent Proteins
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Recombinases
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Green Fluorescent Proteins
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notexin
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Keratins
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beta-Galactosidase