Evaluation of Clonorchis sinensis recombinant 7-kilodalton antigen for serodiagnosis of clonorchiasis

Clin Diagn Lab Immunol. 2004 Jul;11(4):814-7. doi: 10.1128/CDLI.11.4.814-817.2004.

Abstract

The diagnostic applicability of the Clonorchis sinensis recombinant 7-kDa protein was evaluated. In enzyme-linked immunosorbent assays and immunoblots, the protein showed high sensitivities (81.3 and 71.9%, respectively) and specificities (92.6 and 89.7%, respectively) for sera obtained from various helminthic infections. Some paragonimiasis sera showed cross-reactions. The antigen might be valuable in the serodiagnosis of human clonorchiasis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Protozoan / immunology
  • Antigens, Protozoan / immunology*
  • Clonorchiasis / diagnosis*
  • Clonorchiasis / immunology
  • Clonorchis sinensis / immunology
  • Cross Reactions / immunology
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoblotting
  • Parasitic Diseases / diagnosis
  • Parasitic Diseases / immunology
  • Recombinant Proteins / immunology*
  • Sensitivity and Specificity
  • Serologic Tests* / methods

Substances

  • Antibodies, Protozoan
  • Antigens, Protozoan
  • Recombinant Proteins