Phosphoglucomutase 1: complete human and rabbit mRNA sequences and direct mapping of this highly polymorphic marker on human chromosome 1

Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):411-5. doi: 10.1073/pnas.89.1.411.

Abstract

A cDNA clone encoding the mRNA for the highly polymorphic human enzyme phosphoglucomutase 1 (PGM1; EC 5.4.2.2) has been isolated and characterized. This was achieved indirectly by first isolating a rabbit cDNA from an expression library using anti-rabbit PGM antibodies. A comparison of the nucleotide sequences shows that the homologies between human and rabbit PGM1 mRNAs are 92% and 97% for the coding nucleotide sequence and the amino acid sequence, respectively. The derived rabbit amino acid sequence is in complete agreement with the published protein sequence for rabbit muscle PGM. A physical localization of the human PGM1 gene to chromosome 1p31 has been determined by in situ hybridization. Analysis of DNA from a wide variety of vertebrates indicates a high level of PGM1 sequence conservation during evolution.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1*
  • Cloning, Molecular
  • DNA / genetics
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oligodeoxyribonucleotides / chemistry
  • Phosphoglucomutase / genetics*
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • Rabbits
  • Sequence Alignment

Substances

  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • DNA
  • Phosphoglucomutase

Associated data

  • GENBANK/M83088