Effect of overexpression of protective genes on mitochondrial function of stressed astrocytes

J Bioenerg Biomembr. 2004 Aug;36(4):313-5. doi: 10.1023/B:JOBB.0000041760.23917.e1.

Abstract

Antiapoptotic members of the Bcl-2 family have been shown to reduce ischemic brain injury in vivo and in vitro. Understanding early changes in respiration are important in understanding the cells response to stress and the mechanisms of protection afforded by overexpression of protective genes. This mini-review summarizes current knowledge regarding early responses of astrocytes to ischemia-like stress and the effects of overexpression of protective Bcl-2 family genes on astrocyte mitochondrial function. Overexpression of Bcl-x(L) improves mitochondrial respiratory function, normalizes mitochondrial membrane potential, and reduces production of free radicals early after the imposition of a stress in primary cultured murine astrocytes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Apoptosis / physiology*
  • Astrocytes / metabolism*
  • Brain / metabolism*
  • Cytochromes c / metabolism*
  • Gene Expression Regulation / physiology
  • Humans
  • Hypoxia-Ischemia, Brain / metabolism
  • Membrane Potentials / physiology
  • Mitochondria / metabolism*
  • Mitochondrial Proteins / metabolism*
  • Neuroprotective Agents / metabolism
  • Oxidative Stress / physiology*
  • Proto-Oncogene Proteins c-bcl-2 / metabolism*
  • bcl-X Protein

Substances

  • BCL2L1 protein, human
  • Mitochondrial Proteins
  • Neuroprotective Agents
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-X Protein
  • Cytochromes c