Fast separation and determination of phenolic compounds by capillary electrophoresis-diode array detection. Application to the characterisation of alperujo after ultrasound-assisted extraction

J Chromatogr A. 2004 Aug 6;1045(1-2):239-46. doi: 10.1016/j.chroma.2004.06.019.

Abstract

A dynamic approach has been proposed for the ultrasound-assisted extraction of twenty phenolic compounds from alperujo, a semisolid waste from the olive oil industry, that is a representative example of samples with a complex matrix. Multivariate methodology was used to carry out a detailed optimisation study of both the separation-determination and extraction steps in terms of resolution-analysis time and extraction efficiency, respectively. Consequently, the proposed method was able to extract the target analytes in 13 min; then, after dilution and centrifugation, the extract was injected into the capillary electrophoresis-diode array detection system for individual separation determination in 11 min. No cleanup of the extract was required. This method is less time-consuming, more selective and provides a larger information level than the Folin-Ciocalteau spectrophotometric method. Alperujo was demonstrated to be a powerful source of phenolic compounds, particularly as compared with olive oil--8680 versus 50-1200 microg/g.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Capillary / methods*
  • Olive Oil
  • Phenols / analysis*
  • Plant Oils / chemistry*
  • Reproducibility of Results

Substances

  • Olive Oil
  • Phenols
  • Plant Oils