WD repeat-containing mitotic checkpoint proteins act as transcriptional repressors during interphase

FEBS Lett. 2004 Sep 24;575(1-3):23-9. doi: 10.1016/j.febslet.2004.07.089.

Abstract

WD repeats are implicated in protein-protein interactions and regulate a wide variety of cellular functions, including chromatin remodeling and transcription. The WD repeats of the Bub3 and Cdc20 kinetochore proteins are important for the physical interactions of these proteins with Mad2 and BubR1 to yield a kinetochore protein complex capable of delaying anaphase by inhibiting ubiquitin ligation via the anaphase-promoting complex/cyclosome. Here, we show that Bub3 and Cdc20 form a complex with histone deacetylases; this interaction appears to confer transcriptional repressor activity in a heterologous DNA-binding context. In addition, inhibition of Bub3 and Cdc20 expression significantly impairs interphase cell cycle. These results indicate that Bub3 and Cdc20 play additional roles in the integration of cell cycle arrest as transcriptional repressors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cdc20 Proteins
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Genes, Reporter
  • HeLa Cells
  • Histone Deacetylases / metabolism
  • Humans
  • Interphase / physiology*
  • Macromolecular Substances
  • Mitosis / physiology*
  • Poly-ADP-Ribose Binding Proteins
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins / metabolism*
  • Transcription, Genetic*

Substances

  • BUB3 protein, human
  • Cdc20 Proteins
  • Cell Cycle Proteins
  • Macromolecular Substances
  • Poly-ADP-Ribose Binding Proteins
  • RNA, Small Interfering
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • CDC20 protein, human
  • Histone Deacetylases