An optimized method for the assay of the red blood cell--age-related enzyme aspartate aminotransferase

Lab Hematol. 2004;10(3):144-6. doi: 10.1532/LH96.04047.

Abstract

Three methods of preparation of red blood cell concentrate for erythrocyte aspartate aminotransferase measurement were compared: (1) filtration of whole blood through a cellulose column (n = 36); (2) washing of whole blood and aspiration of buffy coat after centrifugation (n = 48); (3) optimized method with washing without aspiration of buffy coat (n = 229).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspartate Aminotransferases / analysis*
  • Biological Assay / methods*
  • Cell Separation / methods
  • Cellular Senescence*
  • Erythrocyte Transfusion
  • Erythrocytes / enzymology*
  • Humans
  • Leukocyte Reduction Procedures / methods
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Aspartate Aminotransferases