Spatially resolved electronic detection of biopolymers

Phys Rev E Stat Nonlin Soft Matter Phys. 2004 Sep;70(3 Pt 1):031906. doi: 10.1103/PhysRevE.70.031906. Epub 2004 Sep 21.

Abstract

An integrated array of field-effect transistor structures is used to detect two oppositely charged biopolymers: poly(L-lysine) and DNA. Local deposition of polymer solutions on part of the array induces sizeable variations in the dc current-voltage characteristics of the transistors exposed to the molecular charge. The whole transistor array is measured in the presence of a common electrolyte. Differential signals are studied as a function of electrolyte salt and polymer concentrations. The measurements provide information on the interface electrostatic potentials of the (semiconductor/biopolymer/electrolyte) system and the experimental data are compared to an analytical model which accounts for screening of the adsorbed charge by mobile ions.

Publication types

  • Comparative Study
  • Evaluation Study
  • Validation Study

MeSH terms

  • Biopolymers / analysis
  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • DNA / analysis*
  • Electrochemistry / instrumentation*
  • Electrochemistry / methods
  • Electrodes
  • Equipment Design
  • Equipment Failure Analysis
  • Oligonucleotide Array Sequence Analysis / instrumentation*
  • Oligonucleotide Array Sequence Analysis / methods
  • Polylysine / analysis*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Transistors, Electronic*

Substances

  • Biopolymers
  • Polylysine
  • DNA