A direct quantitative analysis method for monitoring biogenic volatile organic compounds released from leaves of Pelargonium hortorum in situ

Anal Bioanal Chem. 2004 Dec;380(7-8):950-7. doi: 10.1007/s00216-004-2874-9. Epub 2004 Nov 5.

Abstract

A direct quantitative method is presented that is based upon the use of multiple headspace solid phase microextraction (HS-SPME) to monitor biogenic volatile organic compounds (BVOCs) released from a living leaf of Pelargonium hortorum in situ. Seventeen BVOCs were detected by GC-MS after a single SPME extraction using a CAR/DVB/PDMS fibre. An internal standard was employed to determine the absolute amounts of seven terpenoid compounds released from a P. hortorum leaf. The quantitative analysis was performed over two days, with extraction preformed for 20 min every 3 h. The amount of volatiles extracted varied with the time of day, with two maxima recorded at 14:00 (day 1) and 17:00 (day 2), corresponding to 236 and 277 ng of the seven terpenoids recorded, respectively. These results indicate that multiple HS-SPME in combination with an internal standard is a simple, quick, and quantitative technique for analysising BVOC emissions from a live plant sample.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calibration
  • Chemistry Techniques, Analytical / methods
  • Organic Chemicals / analysis*
  • Pelargonium / chemistry*
  • Plant Leaves / chemistry*
  • Regression Analysis
  • Solvents / chemistry
  • Time Factors
  • Volatilization

Substances

  • Organic Chemicals
  • Solvents