Pan-cytokeratin immunoexpression in Wilms' tumors: a simple approach for understanding tumor epithelial differentiation

Sao Paulo Med J. 2004 Jul 1;122(4):181-3. doi: 10.1590/s1516-31802004000400011. Epub 2004 Nov 9.

Abstract

Wilms' tumor is one of the most common solid tumors in children and is an interesting model for understanding the pathogenesis of embryonal tumors. Cytokeratins are intracellular fibrous proteins present in tissue of epithelial origin. The immunoexpression of the pan-cytokeratin AE1AE3 was studied in paraffin-embedded tissue sections from 24 Wilms' tumors (12 with nephrogenic rests) and also tissue samples from 15 corresponding normal kidneys, to evaluate epithelial differentiation in the genesis of Wilms' tumor. We observed that the intensity of the expression of AE1AE3 in the epithelial component of Wilms' tumors directly correlated with the degree of maturity of the epithelial structures correspondent to the collecting ducts.

Os tumores de Wilms são alguns dos mais freqüentes tumores sólidos da infância e reconhecidamente urn modelo para a compreensão da patogênese dos tumores embrionários. As citoqueratinas são proteínas intracelulares presentes em tecidos de origem epitelial. Estudamos a imunoexpressão da pan-citoqueratina AE1AE3 em 24 tumores de Wilms, dentre ao quais 15 continham também tecidos renais não-neoplásicos e 12 apresentavam restos nefrogênicos em blocos de parafina, para avaliar a diferenciação epitelial no desenvolvimento dos tuniores de Wilms. Observamos aumento na intensidade de expressão de AE1AE3 no componente epitelial dos tuniores de Wilms diretamente relacionado ao grau de maturação das estruturas epiteliais correspondentes aos ductos coletores.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Immunohistochemistry
  • Keratins / metabolism*
  • Kidney Neoplasms / metabolism
  • Kidney Neoplasms / pathology*
  • Wilms Tumor / metabolism
  • Wilms Tumor / pathology*

Substances

  • Keratins