Successful utilization of lyophilized lipoprotein(a) as a biological reagent

Lipids. 2004 Jun;39(6):589-93. doi: 10.1007/s11745-004-1268-5.

Abstract

Lipoprotein(a) [Lp(a)] represents a class of lipoprotein particles having as a protein moiety apoB-100 linked by a single disulfide bond to apolipoprotein(a) [apo(a)], a multikringle structure with a high degree of homology with plasminogen. A recognized feature of Lp(a) is its instability on storage caused by attendant protein and lipid modifications that affect the structural, functional, and immunological properties of this lipoprotein. Here we present data showing that, under appropriate conditions of cryopreservation, Lp(a) retains the properties of the freshly isolated product, and we provide examples supporting the stability of this cryopreserved product as a primary standard in immunoassay settings and in cell culture systems.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cryopreservation / methods
  • Drug Stability
  • Freeze Drying
  • Indicators and Reagents
  • Lipoprotein(a) / standards*
  • Methods
  • Reference Standards

Substances

  • Indicators and Reagents
  • Lipoprotein(a)