Structure-activity relationships of aminoglycoside-arginine conjugates that bind HIV-1 RNAs as determined by fluorescence and NMR spectroscopy

FEBS Lett. 2004 Nov 19;577(3):415-21. doi: 10.1016/j.febslet.2004.10.038.

Abstract

We present here a new set of aminoglycoside-arginine conjugates (AACs) that are either site-specific or per-arginine conjugates of paromomycin, neamine, and neomycin B as well as their structure-activity relationships. Their binding constants (KD) for TAR and RRE RNAs, measured by fluorescence anisotropy, revealed dependence on the number and location of arginines in the different aminoglycoside conjugates. The binding affinity of the per-arginine aminoglycosides to TAR is higher than to RRE, and hexa-arginine neomycin B is the most potent binder (KD=5 and 23 nM, respectively). The 2D TOCSY NMR spectrum of the TAR monoarginine-neomycin complex reveals binding at the bulge region of TAR.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aminoglycosides / metabolism*
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / metabolism*
  • Arginine / chemistry
  • Binding Sites
  • Fluorescence Polarization*
  • HIV-1 / genetics*
  • Molecular Structure
  • Neomycin / chemistry
  • Neomycin / metabolism
  • Nuclear Magnetic Resonance, Biomolecular*
  • Paromomycin / chemistry
  • Paromomycin / metabolism
  • RNA, Viral / metabolism*
  • Structure-Activity Relationship

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • RNA, Viral
  • Paromomycin
  • Arginine
  • Neomycin