Loss of annexin A1 expression in human breast cancer detected by multiple high-throughput analyses

Dejun Shen; Helena R Chang; Zugen Chen; Jianbo He; Victor Lonsberry; Yahya Elshimali; David Chia; David Seligson; Lee Goodglick; Stanley F Nelson; Jeffrey A Gornbein

doi:10.1016/j.bbrc.2004.10.214

Loss of annexin A1 expression in human breast cancer detected by multiple high-throughput analyses

Biochem Biophys Res Commun. 2005 Jan 7;326(1):218-27. doi: 10.1016/j.bbrc.2004.10.214.

Authors

Dejun Shen¹, Helena R Chang, Zugen Chen, Jianbo He, Victor Lonsberry, Yahya Elshimali, David Chia, David Seligson, Lee Goodglick, Stanley F Nelson, Jeffrey A Gornbein

Affiliation

¹ Gonda/UCLA Breast Cancer Research Laboratory, Department of Surgery, Revlon/UCLA Breast Center, University of California at Los Angeles, David Geffen School of Medicine, Los Angeles, CA 90095, USA.

PMID: 15567174
DOI: 10.1016/j.bbrc.2004.10.214

Abstract

To test the efficacy of combined high-throughput analyses (HTA) in target gene identification, screening criteria were set using >fivefold difference by microarray and statistically significant changes (p<0.01) in SAGE and EST. Microarray analysis of two normal and seven breast cancer samples found 129 genes with >fivefold changes. Further SAGE and EST analyses of these genes identified four qualified genes, ERBB2, GATA3, AGR2, and ANXA1. Their expression pattern was validated by RT-PCR in both breast cell lines and tissue samples. Loss of ANXA1 in breast cancer was further confirmed at mRNA level by Human Breast Cancer Tissue Profiling Array and at protein level by immunohistochemical staining. This study demonstrated that combined HTA effectively narrowed the number of genes for further study, while retaining the sensitivity in identifying biologically important genes such as ERBB2 and ANXA1. A distinctive loss of ANXA1 in breast cancer suggests its involvement in maintaining normal breast biology.

Publication types

Clinical Trial
Comparative Study
Controlled Clinical Trial
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Validation Study

MeSH terms

Algorithms
Annexin A1 / analysis
Annexin A1 / genetics
Annexin A1 / metabolism*
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism*
Breast Neoplasms / classification
Breast Neoplasms / diagnosis*
Breast Neoplasms / genetics
Breast Neoplasms / metabolism*
Electrophoresis, Polyacrylamide Gel / methods
Expressed Sequence Tags
Female
Gene Expression Profiling / methods*
Humans
Oligonucleotide Array Sequence Analysis / methods*
Reproducibility of Results
Sensitivity and Specificity
Sequence Analysis, Protein / methods*

Substances

Annexin A1
Biomarkers, Tumor

Abstract

Publication types

MeSH terms

Substances

Grants and funding