Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4

Mikako Ito; Tomohiko Takasaki; Ken-Ichiro Yamada; Reiko Nerome; Shigeru Tajima; Ichiro Kurane

doi:10.1128/JCM.42.12.5935-5937.2004

Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4

J Clin Microbiol. 2004 Dec;42(12):5935-7. doi: 10.1128/JCM.42.12.5935-5937.2004.

Authors

Mikako Ito¹, Tomohiko Takasaki, Ken-Ichiro Yamada, Reiko Nerome, Shigeru Tajima, Ichiro Kurane

Affiliation

¹ Department of Virology 1, National Institute of Infectious Disease, 1-23-1 Toyama, Shinjyuku-ku, Tokyo 162-8640, Japan.

Abstract

The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Dengue / virology*
Dengue Virus / classification
Dengue Virus / genetics
Dengue Virus / isolation & purification*
Fluorescent Dyes*
Humans
RNA, Viral / blood
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Taq Polymerase*

Substances

Fluorescent Dyes
RNA, Viral
Taq Polymerase