A leader intron and 115-bp promoter region necessary for expression of the carnation S-adenosylmethionine decarboxylase gene in the pollen of transgenic tobacco

FEBS Lett. 2004 Dec 17;578(3):229-35. doi: 10.1016/j.febslet.2004.11.005.

Abstract

The expression of CSDC9 encoding S-adenosylmethionine decarboxylase (SAMDC) is developmentally and spatially regulated in carnation. To examine the regulation of the SAMDC gene, we analyzed the spatial expression of CSDC9 with a 5'-flanking beta-glucuronidase fusion in transgenic tobacco plants. GUS was strongly expressed in flower, pollen, stem and vein of cotyledons. Expression in both anther and stigma was under developmental control; analysis of a series of mutants with deletions of the 5'-flanking region demonstrated differential activation in petal, anther, stigma and pollen grains. All the major cis-regulatory elements required for pollen-specific transcription were located in the upstream region between -273 and -158. This region contains four putative elements related to gibberellin induction (pyrimidine boxes, TTTTTTCC and CCTTTT) and pollen-specific expression (GTGA and AGAAA). In addition, the first 5'-leader intron was necessary for tissue-specific expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Flanking Region
  • Adenosylmethionine Decarboxylase / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Plant / chemistry
  • DNA, Plant / genetics
  • DNA, Plant / isolation & purification
  • Dianthus / anatomy & histology
  • Dianthus / chemistry
  • Dianthus / genetics*
  • Flowers / genetics
  • Flowers / growth & development
  • Gene Expression Regulation, Enzymologic*
  • Gene Expression Regulation, Plant*
  • Genes, Plant*
  • Glucuronidase / metabolism
  • Introns*
  • Molecular Sequence Data
  • Nicotiana / genetics
  • Open Reading Frames
  • Plant Leaves / cytology
  • Plant Leaves / genetics
  • Plant Leaves / growth & development
  • Plants, Genetically Modified
  • Plants, Toxic
  • Plasmids
  • Pollen / cytology
  • Pollen / genetics*
  • Promoter Regions, Genetic*
  • RNA, Messenger / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Deletion
  • TATA Box

Substances

  • DNA, Plant
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Glucuronidase
  • Adenosylmethionine Decarboxylase