Abstract
The expression of CSDC9 encoding S-adenosylmethionine decarboxylase (SAMDC) is developmentally and spatially regulated in carnation. To examine the regulation of the SAMDC gene, we analyzed the spatial expression of CSDC9 with a 5'-flanking beta-glucuronidase fusion in transgenic tobacco plants. GUS was strongly expressed in flower, pollen, stem and vein of cotyledons. Expression in both anther and stigma was under developmental control; analysis of a series of mutants with deletions of the 5'-flanking region demonstrated differential activation in petal, anther, stigma and pollen grains. All the major cis-regulatory elements required for pollen-specific transcription were located in the upstream region between -273 and -158. This region contains four putative elements related to gibberellin induction (pyrimidine boxes, TTTTTTCC and CCTTTT) and pollen-specific expression (GTGA and AGAAA). In addition, the first 5'-leader intron was necessary for tissue-specific expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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5' Flanking Region
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Adenosylmethionine Decarboxylase / genetics*
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA, Plant / chemistry
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DNA, Plant / genetics
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DNA, Plant / isolation & purification
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Dianthus / anatomy & histology
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Dianthus / chemistry
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Dianthus / genetics*
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Flowers / genetics
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Flowers / growth & development
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Gene Expression Regulation, Enzymologic*
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Gene Expression Regulation, Plant*
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Genes, Plant*
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Glucuronidase / metabolism
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Introns*
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Molecular Sequence Data
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Nicotiana / genetics
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Open Reading Frames
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Plant Leaves / cytology
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Plant Leaves / genetics
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Plant Leaves / growth & development
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Plants, Genetically Modified
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Plants, Toxic
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Plasmids
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Pollen / cytology
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Pollen / genetics*
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Promoter Regions, Genetic*
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RNA, Messenger / genetics
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Recombinant Fusion Proteins / metabolism
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Sequence Deletion
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TATA Box
Substances
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DNA, Plant
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RNA, Messenger
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Recombinant Fusion Proteins
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Glucuronidase
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Adenosylmethionine Decarboxylase