Production and in vivo effects of chemokines CXCL1-3/KC and CCL2/JE in a model of inflammatory angiogenesis in mice

Inflamm Res. 2004 Oct;53(10):576-84. doi: 10.1007/s00011-004-1299-4.

Abstract

Objective: Using the murine sponge model, we investigated the temporal relationship between angiogenesis, leukocyte accumulation and endogenous generation of the pro-inflammatory chemokines CXCL1-3/KC and CCL2/JE. Furthermore, the effects of exogenous administration of these chemokines were studied.

Methods: Angiogenesis in the implants was assessed by measuring the hemoglobin content (vascular index) and leukocyte accumulation quantified by evaluating MPO and NAG enzyme activities.

Results: A progressive increase in hemoglobin content and in enzymatic activities was observed during the whole period. The levels of CXCL1-3/KC and CCL2/JE in the implants peaked at days 7 and 1, respectively. Exogenous administration of CXCL1-3/KC (100 ng/day intra-implant) applied at days 1-3 resulted in increased neovascularization and macrophage accumulation. Intra-implant injections of CCL2/JE (100 ng/day) also resulted in increased angiogenesis and macrophage accumulation.

Conclusions: These results demonstrated that the chemokines, CXCL1-3/KC and CCL2/JE, are generated within the sponge compartment and that neovascularization and inflammatory cells influx can be modulated by exogenous administration of the chemokines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosaminidase / chemistry
  • Animals
  • Chemokine CCL2 / biosynthesis*
  • Chemokine CXCL1
  • Chemokines / metabolism
  • Chemokines, CXC / biosynthesis*
  • Disease Models, Animal
  • Enzyme-Linked Immunosorbent Assay
  • Hemoglobins / metabolism
  • Immunohistochemistry
  • Inflammation
  • Intercellular Signaling Peptides and Proteins / biosynthesis*
  • Kinetics
  • Leukocytes / cytology
  • Leukocytes / immunology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Neovascularization, Pathologic*
  • Neutrophils / metabolism
  • Peroxidase / metabolism
  • Recombinant Proteins / chemistry
  • Time Factors

Substances

  • Chemokine CCL2
  • Chemokine CXCL1
  • Chemokines
  • Chemokines, CXC
  • Cxcl1 protein, mouse
  • Hemoglobins
  • Intercellular Signaling Peptides and Proteins
  • Recombinant Proteins
  • Peroxidase
  • Acetylglucosaminidase